The Merbl Lab
PTM Profiling: revealing a novel dimension in molecular and clinical diagnosis
With a motivation to decode the regulatory code of Post-Translational Modifications (PTMs), our lab has developed the PTM profiling approach which allows for high-throughput and sensitive detection of multiple ubiquitin and ubiquitin-like modifiers such as SUMO, FAT10, ISG15 and others, in various cellular systems a well as in clinical samples. We utilize this system to map the PTM landscape in health and disease focusing primarily on cancer and autoimmunity.
Cellular proteostasis in health and disease
Cellular proteolysis is involved in essentially every aspect of cell biology. We are interested in understanding the principles underlying the fine-tuning regulation of proteasome activity, substrate specificity and the dynamic rearrangements of the cellular proteome in cancer and immune regulation.
Using our novel Proteasomal Footprinting approach through a method we have termed Mass Spectrometry analysis of Proteolytic Peptides (MAPP) we can examine proteasome-cleaved peptides in cells, in peptide resolution. Utilizing this system we are now exploring the cellular degradome and resulting proteolytic landscape in numerous human disease ranging from cancer to neurodegeneration and autoimmunity.